MYOG Validated Chimera RNAi
产品名称: MYOG Validated Chimera RNAi
英文名称: MYOG Validated Chimera RNAi
产品编号: H00004656-R01V
产品价格: 0
产品产地: 台湾
品牌商标: Abnova
更新时间: null
使用范围: null
亚诺法生技股份有限公司(Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
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- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Homo sapiens myogenin (myogenic factor 4) (MYOG), mRNA.
- Reactivity:
- Human
- Storage Instruction:
- Store at -20°C, do not exceed 4 - 5 freeze-thaw cycles to ensure product integrity.
- Supplied Product:
- DEPC water
- Target Refseq:
- NM_002479
- Note:
- Position of the Chimera RNAi.
-
- Publication Reference
- 1.
- dsCheck: highly sensitive off-target search software for double-stranded RNA-mediated RNA interference.
Naito Y, Yamada T, Matsumiya T, Ui-Tei K, Saigo K, Morishita S.Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W589-91.
- 2.
- Functional dissection of siRNA sequence by systematic DNA substitution: modified siRNA with a DNA seed arm is a powerful tool for mammalian gene silencing with significantly reduced off-target effect.
Ui-Tei K, Naito Y, Zenno S, Nishi K, Yamato K, Takahashi F, Juni A, Saigo K.Nucleic Acids Res. 2008 Apr;36(7):2136-51. Epub 2008 Feb 11.
- 3.
- Guidelines for the selection of highly effective siRNA sequences for mammalian and chick RNA interference.
Ui-Tei K, Naito Y, Takahashi F, Haraguchi T, Ohki-Hamazaki H, Juni A, Ueda R, Saigo K.Nucleic Acids Res. 2004 Feb 9;32(3):936-48. Print 2004.
- 4.
- siDirect: highly effective, target-specific siRNA design software for mammalian RNA interference.
Naito Y, Yamada T, Ui-Tei K, Morishita S, Saigo K.Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W124-9.
- Applications
- RNAi Knockdown (Antibody validated)
- Western blot analysis of MYOG over-expressed 293 cell line, cotransfected with MYOG Validated Chimera RNAi ( Cat # H00004656-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MYOG monoclonal antibody (M01), clone 2B7 (Cat # H00004656-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- Entrez GeneID:
- 4656
- Gene Name:
- MYOG
- Gene Alias:
- MYF4,MYOGENIN,bHLHc3
- Gene Description:
- myogenin (myogenic factor 4)
- Omim ID:
- 159980
- Gene Ontology:
- Hyperlink
- Gene Summary:
- Myogenin is a muscle-specific transcription factor that can induce myogenesis in a variety of cell types in tissue culture. It is a member of a large family of proteins related by sequence homology, the helix-loop-helix (HLH) proteins. It is essential for the development of functional skeletal muscle. [provided by RefSeq
- Other Designations:
- Myogenic factor-4; myogenin,OTTHUMP00000039094,myogenic factor 4,myogenin