RPS6KA2 monoclonal antibody (M01), clone 1F6
产品名称： RPS6KA2 monoclonal antibody (M01), clone 1F6
英文名称： RPS6KA2 monoclonal antibody (M01), clone 1F6
产品编号： H00006196-M01
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against a partial recombinant RPS6KA2.
- Immunogen:
- RPS6KA2 (AAH02363, 631 a.a. ~ 733 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
- Sequence:
- YALSGGNWDSISDAAKDVVSKMLHVDPHQRLTAMQVLKHPWVVNREYLSPNQLSRQDVHLVKGAMAATYFALNRTPQAPRLEPVLSSNLAQRRGMKRLTSTRL
- Host:
- Mouse
- Reactivity:
- Human
- Isotype:
- IgG2a Kappa
- Storage Buffer:
- In 1x PBS, pH 7.2
- Storage Instruction:
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.07 KDa) .
- MSDS:
Download
- Applications
- Western Blot (Cell lysate)
- RPS6KA2 monoclonal antibody (M01), clone 1F6 Western Blot analysis of RPS6KA2 expression in Hela NE ( Cat # L013V3 ).
- Protocol Download
- Western Blot (Transfected lysate)
- Western Blot analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody (M01), clone 1F6.
Lane 1: RPS6KA2 transfected lysate(83.2 KDa).
Lane 2: Non-transfected lysate. - Protocol Download
- Western Blot (Recombinant protein)
- Protocol Download
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
enlarge this image- Immunoperoxidase of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3 ug/ml]
- Protocol Download
- Immunofluorescence
enlarge this image- Immunofluorescence of monoclonal antibody to RPS6KA2 on HeLa cell. [antibody concentration 10 ug/ml]
- Protocol Download
- RNAi Knockdown (Antibody validated)
- Western blot analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi ( Cat # H00006196-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RPS6KA2 monoclonal antibody (M01) clone 1F6 (Cat # H00006196-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Protocol Download
- In situ Proximity Ligation Assay (Cell)
- Proximity Ligation Analysis of protein-protein interactions between MAPK3 and RPS6KA2 HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Application Image
- Western Blot (Cell lysate)
- enlarge
- Western Blot (Transfected lysate)
- enlarge
- Western Blot (Recombinant protein)
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- enlarge
- Immunofluorescence
- enlarge
- ELISA
- RNAi Knockdown (Antibody validated)
- enlarge
- In situ Proximity Ligation Assay (Cell)
- enlarge
- Entrez GeneID:
- 6196
- GeneBank Accession#:
- BC002363
- Protein Accession#:
- AAH02363
- Gene Name:
- RPS6KA2
- Gene Alias:
- HU-2,MAPKAPK1C,RSK,RSK3,S6K-alpha,S6K-alpha2,p90-RSK3,pp90RSK3
- Gene Description:
- ribosomal protein S6 kinase, 90kDa, polypeptide 2
- Omim ID:
- 601685
- Gene Ontology:
- Hyperlink
- Gene Summary:
- This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq
- Other Designations:
- ribosomal S6 kinase 3,ribosomal protein S6 kinase alpha 2,ribosomal protein S6 kinase, 90kD, polypeptide 2
- Related Disease