DYKDDDDK tag monoclonal antibody, clone 29E4.G7.H11
产品名称： DYKDDDDK tag monoclonal antibody, clone 29E4.G7.H11
英文名称： DYKDDDDK tag monoclonal antibody, clone 29E4.G7.H11
产品编号： MAB2094
产品价格： null
产品产地： 台湾
品牌商标： Abnova
更新时间： null
使用范围：
亚诺法生技股份有限公司（Abnova)
- 联系人 :
- 地址 : 台湾台北市内湖区洲子街 108 号 9 楼
- 邮编 : 11493
- 所在区域 : 台湾
- 电话 : +886-920**1152 点击查看
- 传真 : 点击查看
- 邮箱 : sales@abnova.com.tw
- Specification
- Product Description:
- Mouse monoclonal antibody raised against synthetic peptides of FLAG epitope tag.
- Immunogen:
- A synthetic peptide corresponding to FLAG epitope tag.
- Host:
- Mouse
- Form:
- Liquid
- Isotype:
- IgG
- Storage Buffer:
- In 20 mM potassium phosphate buffer, 150 mM NaCl, pH 7.2 (0.01% sodium azide)
- Storage Instruction:
- Store at 4°C. For long term storage store at -20°C.
Aliquot to avoid repeated freezing and thawing.
- Quality Control Testing:
- Antibody Reactive Against Synthetic Peptide.
- Recommend Usage:
- ELISA (1:160000)
Western Blot (1:2000-1:20000)
The optimal working dilution should be determined by the end user.
- Note:
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Publication Reference
- 1.
- A strategy for the expression of recombinant proteins traditionally hard to purify.
Frank EG, McDonald JP, Karata K, Huston D, Woodgate R.Anal Biochem. 2012 Jul 22.
- 2.
- Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibodies M1, M2 and M5.
Slootstra JW, Kuperus D, Pluckthun A, Meloen RH.Mol Divers. 1997;2(3):156-64.
- 3.
- Double tagging recombinant A1- and A2A-adenosine receptors with hexahistidine and the FLAG epitope. Development of an efficient generic protein purification procedure.
Robeva AS, Woodard R, Luthin DR, Taylor HE, Linden J.Biochem Pharmacol. 1996 Feb 23;51(4):545-55.
- 4.
- Vectors for expression and secretion of FLAG epitope-tagged proteins in mammalian cells.
Chubet RG, Brizzard BL.Biotechniques. 1996 Jan;20(1):136-41.
- Applications
- Western Blot (Recombinant protein)
- DYKDDDDK tag monoclonal antibody, clone 29E4.G7.H11 (Cat # MAB2094) to detect FLAG™ conjugated proteins detects both C terminal linked and N terminal linked FLAG™ tagged recombinant proteins by western blot.
