分子量:72.7  kDa

纯度:>95% as determined by SDS-PAGE.

内毒素:Less than 1.0 EU per μg of the Human Semaphorin 4A, His Tag by the LAL method.

Buffer:PBS, pH7.4

生物活性:Measured by its ability to bind biotinylated mouse SEMA4D in a functional ELISA.

产品特性:Human Semaphorin 4A, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 72.7  kDa. The predicted N-terminus is Gly 33. The reducing (R) protein migrates as 70-80 kDa in SDS-PAGE  due to glycosylation.

产品背景:Semaphorin-4A (SEMA4A) is also known as Semaphorin-B (SEMAB), which belongs to the semaphorin family. SEMA4A contains one Ig-like C2-type (immunoglobulin-like) domain, one PSI domain and one sema domain. SEMA4A is cell surface receptor for PLXNB1, PLXNB2, PLXNB3 and PLXND1 that plays an important role in cell-cell signaling. SEMA4A plays a role in priming antigen-specific T-cells, promotes differentiation of Th1 T-helper cells, and thereby contributes to adaptive immunity. SEMA4A also promotes phosphorylation of TIMD2 and inhibits angiogenesis. SEMA4A promotes axon growth cone collapse and inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons.